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IL‐23 in gingival tissue by western blotting. Representative blot of HS ( n = 4) and P ( n = 4) samples. The expression of IL‐23 in gingival tissue was evaluated using the western blotting technique and was expressed as ARU. Representative bands and graphs of IL‐23 (19 kDa) and β‐actin (43 kDa). ARU, area relative units; HS, healthy subjects; MW, molecular weight; P, periodontitis. Medians and the maximum and minimum represent data (Mann–Whitney U statistical analysis).

Journal: International Journal of Dentistry

Article Title: Overexpression of the IL‐23/IL‐17A Axis and Their Receptors (IL‐23R and IL‐17RA) in Gingival Tissue of Patients With Periodontitis

doi: 10.1155/ijod/8893504

Figure Lengend Snippet: IL‐23 in gingival tissue by western blotting. Representative blot of HS ( n = 4) and P ( n = 4) samples. The expression of IL‐23 in gingival tissue was evaluated using the western blotting technique and was expressed as ARU. Representative bands and graphs of IL‐23 (19 kDa) and β‐actin (43 kDa). ARU, area relative units; HS, healthy subjects; MW, molecular weight; P, periodontitis. Medians and the maximum and minimum represent data (Mann–Whitney U statistical analysis).

Article Snippet: The membranes were then incubated with the appropriate primary antibody overnight at 4°C: mouse monoclonal anti‐human IL‐17 (G‐4) IgG2B diluted 1:500 (sc‐374218, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐human IL‐23 (c‐3) monoclonal mouse IgG1 diluted 1:500 (sc‐271279, Santa Cruz Biotechnology), anti‐human IL‐17RA (D1Y4C) monoclonal rabbit IgG diluted 1:1000 (12661, Cell Signaling Technology, Danvers MA, USA), anti‐human IL‐23R (A48090) polyclonal rabbit IgG diluted 1:300 (Antibodies.com, Cambridge, UK), and β‐actin (C4) mouse monoclonal IgG1k diluted 1:1000 (sc‐47778, Santa Cruz Biotechnology).

Techniques: Western Blot, Expressing, Molecular Weight, MANN-WHITNEY

Correlation between IL‐23/IL‐17A axis molecules and IL‐23R and IL‐17RA receptors with clinical parameters in gingival tissue. The Spearman correlation test was used to analyze the values of periodontal clinical parameters and the concentrations of IL‐23, IL‐17A, IL‐23R, and IL‐17RA. BoP%, percentage of bleeding on probing; CAL, clinical attachment loss; IL, interleukin; PD, probing depth; RBL%, radiographic bone loss, ∗ p ≤ 0.05, ∗∗ p ≤ 0.001.

Journal: International Journal of Dentistry

Article Title: Overexpression of the IL‐23/IL‐17A Axis and Their Receptors (IL‐23R and IL‐17RA) in Gingival Tissue of Patients With Periodontitis

doi: 10.1155/ijod/8893504

Figure Lengend Snippet: Correlation between IL‐23/IL‐17A axis molecules and IL‐23R and IL‐17RA receptors with clinical parameters in gingival tissue. The Spearman correlation test was used to analyze the values of periodontal clinical parameters and the concentrations of IL‐23, IL‐17A, IL‐23R, and IL‐17RA. BoP%, percentage of bleeding on probing; CAL, clinical attachment loss; IL, interleukin; PD, probing depth; RBL%, radiographic bone loss, ∗ p ≤ 0.05, ∗∗ p ≤ 0.001.

Article Snippet: The membranes were then incubated with the appropriate primary antibody overnight at 4°C: mouse monoclonal anti‐human IL‐17 (G‐4) IgG2B diluted 1:500 (sc‐374218, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti‐human IL‐23 (c‐3) monoclonal mouse IgG1 diluted 1:500 (sc‐271279, Santa Cruz Biotechnology), anti‐human IL‐17RA (D1Y4C) monoclonal rabbit IgG diluted 1:1000 (12661, Cell Signaling Technology, Danvers MA, USA), anti‐human IL‐23R (A48090) polyclonal rabbit IgG diluted 1:300 (Antibodies.com, Cambridge, UK), and β‐actin (C4) mouse monoclonal IgG1k diluted 1:1000 (sc‐47778, Santa Cruz Biotechnology).

Techniques: